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7-AAD is a fluorescent DNA binding dye that is membrane impermeant and therefore generally excluded from live cells and early apoptotic cells, but stains necrotic and late apoptotic cells with compromised membrane integrity.Buy 7-add Powder
Selective detection of dead cells by flow cytometry in the PE-Cy®5/PerCP channel
Perform cell cycle profiling by flow cytometry in fixed/permeabilized cells
λEx/λEm (with DNA) = 546/647 nm (7-AAD can also be excited by the 488 or 532 laser lines)
Orange red solid soluble in DMF or DMSO
Store at -20°C and protect from light, especia
7-AAD bound to DNA has a large Stokes shift with excitation/emission at 546/647 nm. The dye can be excited with either the 488 nm or 532 nm laser lines, and detected in the PE-Cy®5/PerCP flow cytometry channel. 7-AAD is widely used for live/dead cell discrimination. It also can be used in fixed and permeabilized cells for cell cycle profiling by DNA content analysis using flow cytometry. 7-AAD intercalates selectively at GC-rich regions of DNA, making it dye useful for chromosome banding studies. We offer 7-AAD as a solid powder (catalog no. 40037) or as a solution in 1 mg/mL DMSO:Water (1:1) (catalog no. 40084).The related compound actinomycin D is nonfluorescent, but binds DNA in the same way as 7-AAD. Its absorbance changes when bound to DNA, and it can be used as a stain in conventional transmission microscopy. Order 7-ADD POWDER AThttps://authenticchemicals.com/product/Buy 7-add Powder/